|
KMID : 0613820080180020284
|
|
Journal of Life Science
2008 Volume.18 No. 2 p.284 ~ p.286
|
|
|
Development of Molecular Marker to Distinguish Octopus minor Sasaki Caught in Korea and that in China
|
|
Kim Joo-Il
Oh Taeg-Yun
Yang Won-Seok
Cho Eun-Seob
|
|
|
Abstract
|
|
|
|
Octopus minor (O. minor) is widely distributed along the coastal regions of Korea, but most of them are caught in southern waters which are associated with one of the important fisheries stock. At present, O. minor from China has been introduced to the fishery markets in Korea. Here, we attempt to discriminate their origin for Korea or China using molecular techniques. Based on the O. minor mitochondrial DNA sequence, we developed a PCR-based origin discrimination system. The assay specificity was assessed by testing four individuals of O. minor from Sangdong, China, as well as 20 additional O. minor from Namhae, Muan, Yeosu and Jindo, Korea. Only four isolates of O. minor originated from China tested as positive in our distinction system. All PCR-positive products yielded identical sequences from Chinese O. minor, whereas Korean O. minor appeared to be PCR amplification. This result suggested that the primers used in this study are O. minor species specific, especially originated from China. The detection system appeared to be positive results in the use of 0.1 ng of Chinese O. minor DNA as template, however, the Korean O. minor even using of DNA showed no amplification. Consequently, the assay provides a simple, rapid and accurate method for the detection of Chinese O. minor.
|
|
|
KEYWORD
|
|
|
China, detection, DNA primer, Korea, PCR, octopus
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|